Selective venous sampling

Investigation Protocol


To help define the exact location of hormone secretion. This includes sampling of the parathyroid, adrenal, gonadal, pancreatic and other regions.

Patient preparation and precautions

Patients should be fasting from midnight (water only). Patients should bring all their usual medication with them to take after the test, and for documentation.

Full blood count, clotting screen and renal function must be documented as normal prior to the test. Blood should also be taken for 'group and save' prior to the test in case transfusion is required.


Insert a cannula gauge 20 with a three-way tap, and using a syringe or the vacutainer connector system, fill the required bottles for baseline sampling. Many hormones including cortisol and aldosterone may be collected in a plain clotted bottle. However, others including ACTH, metanephrines require an EDTA tube, and some require a lithium heparin tube for example insulin and renin. This forms the t= -15 minute sample. Tubes need to be labelled, any that require it should be centrifuged, separated and frozen before transport to the laboratory.

Repeat this basal sampling after 15 minutes, and label t= 0 minutes.

The radiologist will then perform selective venous sampling of the reqested areas, via a femoral puncture. After successful cannulation, infusions may be administered for example of CRH or calcium, and multiple samples taken.  Generally, peripheral samples are always required at baseline and at any additional time points. The utmost care is required in sample labelling and handling in all cases. Following the procedure, and decannulation by the radiologist, apply firm pressure on the puncture sites for approximately 5 minutes. Routine observations should be performed every 15 minutes for the first hour, and the patient should lie flat for the first 30 minutes, or as advised by the radiologist.

The detailed protocol for selective venous sampling is beyond the scope of this guide.


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Patient information sheet

Inferior petrosal sinus sampling

Your doctor has arranged for you to undergo a test called selective venous sampling. The purpose of this is to help us to find out where exactly in your body is producing excessive hormone. This involves taking a series of blood samples from the veins draining the suspected region.

It is normal for patients to be admitted to hospital early on the day of the procedure and to go home later in the day if they are well, sometimes this is not until the evening. There is no special preparation before the procedure apart from a blood test which you must have done about two to three days before the appointment date. 

Please come to the ward at 8am on the morning of the test. We need you in early to prepare you (get you into a special theatre gown and for a doctor to come and talk to you and examine you). You can eat and drink but it is preferable to have a light breakfast because you will be lying flat on an x-ray table during the procedure and in a bed for a few hours after. 

The procedure is undertaken in the angiography suite (a special x-ray room) by one of our consultant radiologists (x-ray doctor). They will explain what will happen and you will be able to ask questions before you are asked to sign a consent form. This is your agreement to have this procedure.

During the procedure you will be asked to lie flat on a special X-ray table. The skin in both of your groins will be cleaned with cold fluid before you are given an injection of local anaesthetic to numb the area. You should not feel any pain following this, but you will feel some gentle pushing as catheters (special tubes) are inserted into the main vein in each groin. The catheters are then guided through the veins to the region under investigation.

X-rays are taken during this time to make sure that the catheters are being guided to the correct place. Once the catheters are in their final position, this is checked by injecting some x-ray contrast. While this is happening patients have reported experiencing a rushing noise in their ears, which lasts for no more than a second or two.

A series of blood samples are then taken. During this time you may also be given an injection of a hormone, through one of the catheters. Once the necessary blood samples have been obtained the catheters are then removed. The nurse will apply gentle pressure to the groin area for about 5 minutes to help stop any bleeding from the small puncture wounds. You will be asked to remain lying flat for about half an hour before you are returned to the ward and gradually allowed to sit up in bed. Following a period of time you will be able to mobilize, get dressed and if all is satisfactory you will be allowed to go home.

If you have any queries about this procedure, please contact: 

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